منابع مشابه
Yeast Rev1 protein is a G template-specific DNA polymerase.
Rev1 protein of Saccharomyces cerevisiae functions with DNA polymerase zeta in mutagenic trans-lesion synthesis. Because of the reported preferential incorporation of a C residue opposite an abasic site, Rev1 has been referred to as a deoxycytidyltransferase. Here, we use steady-state kinetics to examine nucleotide incorporation by Rev1 opposite undamaged and damaged template residues. We show ...
متن کاملMulti-template polymerase chain reaction
PCR is a formidable and potent technology that serves as an indispensable tool in a wide range of biological disciplines. However, due to the ease of use and often lack of rigorous standards many PCR applications can lead to highly variable, inaccurate, and ultimately meaningless results. Thus, rigorous method validation must precede its broad adoption to any new application. Multi-template sam...
متن کاملTemplate-free, polymerase-free DNA polymerization.
A combination of a DNA ligase and a restriction endonuclease provides a DNA polymerase activity, which might suggest a novel strategy for polymer synthesis.
متن کاملTemplate requirement of maize RNA polymerase.
Maize RNA polymerase utilizes heated deoxyribonucleic acid more effectively than native deoxyribonucleic acid as a template for ribonucleic acid synthesis. A ribonucleic acid-deoxyribonucleic acid hybrid accumulates in the presence of heated deoxyribonucleic acid. The amount of product formed with either native or heat-denatured deoxyribonucleic acid does not exceed the amount of deoxyribonucle...
متن کاملDNA polymerase-mediated DNA synthesis on a TNA template.
TNA, or threose nucleic acid, is capable of Watson-Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA t...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2005
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1712rr2